منابع مشابه
A SPECIFIC, SENSITIVE, AND ONE-STEP EXTRACTION RADIOIMMUNOASSAY OF TESTOSTERONE FOR CLINICAL DIAGNOSIS
Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3- O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure ...
متن کاملRadioimmunoassay for dexamethasone in plasma.
Until recently there has been no adequate clinical method for precisely measuring dexamethasone in blood. Studies of therapeutic dexamethasone concentrations and of its metabolism in plasma have relied on the use of radioactive dexamethasone (1-3). We report here an immunoassay in which antisera obtained from rabbits immunized with a conjugate of dexamethasone and bovine serum albumin (BSA) are...
متن کاملRadioimmunoassay for flupenthixol in plasma.
We describe a radioimmunoassay for the neuroleptic drug flupenthixol, suitable for routine monitoring of its concentration in blood. Antibodies for the assay were raised in a sheep against a 7-carboxyflupenthixol/ovalbumin conjugate. The resulting assay, with [3H] flupenthixol as the label, is capable of detecting 2.0 microgram of flupenthixol per liter, in a 100-microliter plasma sample. The a...
متن کامل[Radioimmunoassay of plasma cortisol].
A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated corti...
متن کاملRadioimmunoassay of plasma testosterone, with use of polyethylene glycol to separate antibody-bound and free hormone.
We have developed a reliable radioimmunoassay for testosterone in plasma, polyethylene glycol ("Carbowax 6000") being used to separate antibody-bound and free hormone. Testosteron is separated from interfering steroids, notably dihydrotestosterone, by liquid-liquid partition chromatography on infusorial earth (Celite). The assay is sensitive (9 pg for standards), precise, and accurate. The lowe...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1972
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1972.10.4.164